PLoS Negl Trop Dis 17(7):Įditor: Esteban Chaves-Olarte, Universidad de Costa Rica, COSTA RICA (2023) Scanning iron response regulator binding sites using Dap-seq in the Brucella genome. Collectively, these results provide valuable insights for the exploration of Brucella iron metabolism.Ĭitation: Zhang H, Sun T, Cao X, Wang Y, Ma Z, Wang Y, et al. In summary, our results identified six genes regulated by the Irr protein that may participate in iron metabolism, and the rirA was identified as a regulon of Irr and it also plays a role in iron metabolism of Brucella. Furthermore, the iron utilization and growth assay experiments confirmed that rirA was involve in iron metabolism and growth of Brucella. The electrophoretic mobility shift assay confirmed that six out of the seven genes, namely rirA (BME_RS13665), membrane protein (BME_RS01725), hypothetical protein (BME_RS09560), ftrA (BME_RS14525), cation-transporting P-type ATPase (zntA) (BME_RS10660), and 2Fe-2S binding protein (BME_RS13655), interact with the Irr protein. A total of seven genes were identified as target genes for Irr in this study and the expression levels of these seven genes was identified using qRT-PCR. Therefore, in the present study, a Dap-seq approach was used to investigate the other iron metabolism genes that are also regulated by the Irr protein in Brucella. Previous studies suggested that the Irr protein has multiple target genes in the Brucella genome that are involved in iron metabolism. In addition, irr mutation significantly reduced iron uptake under iron-limited conditions. melitensis M5-90 under iron-limited conditions. melitensis M5-90 irr mutant was significantly higher than that of B. melitensis M5-90 under normal or iron-sufficient conditions, however, the growth rate of the B. The growth rate of Brucella melitensis M5-90 irr mutant was significantly lower than that of B. Iron response regulator (Irr) is a crucial transcriptional regulator and can affect the growth and iron uptake of Brucella. Iron is an essential element required for all organisms.
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